Table 2.

Examined clinical samples and assay results.

No.	Clinical sample	cobas® SARS-CoV-2 (Roche)		Allplex™ 2019-nCoV Assay (Seegene)			RealStar® SARS-CoV-2 RT-PCR Kit 1.0 (altona)		Xpress Xpert® SARS-CoV-2 (Cepheid)		Vivalytic VRI Panel (Schnelltest COVID-19) (Bosch)
	Initial CT-value (undiluted)*	ORF1a gene CT-value (log10 RNA copies/mL)	E gene CT-value (log10 RNA copies/mL)	E gene (CT-value)	RdRP gene (CT-value)	N gene (CT-value)	S gene (CT-value)	E gene (CT-value)	E gene (CT-value)	N2 gene (CT-value)	E/ORFf1ab gene
1	24.5 (T) ▮	25.65 (6.03)	26.31 (5.58)	22.88	25.11	25.88	21.74	2180	–	–	positive
2	30.2 (T) ▮	31.27 (4.31)	33.64 (3.35)	32.07	35.65	34.27	31.07	3199	33.7	36.1	ø
3	33.8 (T) ▮	31.94 (4.12)	34.19 (3.18)	31.89	34.81	34.61	30.99	3167	32.4	35.4	positive
4	29.2 (S) ▮	30.21 (4.63)	31.31 (4.06)	29.63	31.09	32.64	28.42	2848	30.6	33.1	positive
5	25.2 (T) ▮	30.23 (4.63)	31.06 (4.13)	2808	30.20	30.86	26.80	2685	29.1	31.5	positive
6	22.1 (T) ▮	27.1 (5.58)	27.81 (5.12)	24.28	26.56	272	22.70	22.91	–	–	positive
7	29.37 (N) ▮	33.81 (5.58)	35.67 (2.73)	31.53	33.03	33.66	30.60	31.34	32.5	34.4	ø
8	35.6 (P) △	ø	ø	ø	ø	ø	ø	ø	–	–	ø
9	35.65 (P) □	ø	ø	ø	ø	ø	ø	ø	–	–	ø
10	32.12 (N) ▲	36.91 (2.59)	37.42 (2.19)	ø	ø	37.24	34.4	37.05	–	–	positive
11	ø (S)	ø	ø	ø	ø	ø	ø	ø	ø	ø	ø
12	ø (P)	ø	ø	ø	ø	ø	ø	ø	ø	ø	ø
13	ø (P)	ø	ø	ø	ø	ø	ø	ø	ø	ø	ø
14	ø (P)	ø	ø	ø	ø	ø	ø	ø	–	–	ø
15	ø (P)	ø	ø	ø	ø	ø	ø	ø	–	–	ø

All samples where a signal was detected were considered SARS-CoV-2 positive according to each manufacturer’s interpretation algorithm.

*RdRP-gene specific rRT-PCR.

(T) = tracheal secretion.

(S) = sputum.

(N) = nasal swab.

(P) = pharyngeal swab.

▮ = severe clinical course.

▲ = moderate clinical course.

△ = mild clinical course.

□ = asymptomatic clinical course.

Ø = negative.

- not tested.