Table 3.
– Results overview of the examined assays.
| Examined samples | cobas® SARS-CoV-2 (Roche) | Allplex™ 2019-nCoV Assay (Seegene) | SARS-CoV-2 AMP Kit (Abbott) | RealStar® SARS-CoV-2 RT-PCR Kit 1.0 (altona) | Xpress Xpert® SARS-CoV-2 (Cepheid) | Vivalytic VRI Panel (Schnelltest COVID-19) (Bosch) |
|---|---|---|---|---|---|---|
| Dilution series panel (highest dilution where SARS-CoV-2-RNA was detected in) | 10−5 | 10−9,5 (N gene only) | 10−8.5 | 10−7 (S gene only) |
10−8 (N2 gene only) | 10−4 |
| Selected clinical samples (detected) | ✓(8/8) | ✓(8/8***) | – | ✓(8/8) | ✓(5/5) | (✓)(6/8) |
| EQA samples (correctly determined) | ✓(12/12) | (✓)(11/12) | ✓ (5/5) | (✓)(4/5) | ✓(7/7) | ✓(7/7**) |
| Other Coronaviruses (correctly not detected/identified)**** | ✓(4/4) | ✓(4/4) | – | ✓(4/4) | ✓(2/2) | ✓(4/4*****) |
*only the 10−3 sample was tested.
**one sample was detected as “Sarbeco-related”.
***one sample was only detected in the N gene.
****SARS-CoV-1 (2003 outbreak) excluded.
*****MERS-CoV was detected as “SARS-related”.
- not tested.
✓perfect performance (all samples were determined correctly).
(✓) good performance (nearly all samples were determined correctly).