Table 4.
Base cost (CAD$) of commercial kit and procedure time to conduct extract-free LAMP assay.
| per reaction | per result1 | per 24 | per 96 | |
|---|---|---|---|---|
| WarmStart Colorimetric Kits2 | ∼$2−3 | ∼$10−15 | ∼$150-$230 | ∼$600-$900 |
| Master Mix/Primers | $1.76−2.82 | $8.45−13.53 | $135−216 | $540−860 |
| LAMP Primer Mixes | $0.02−0.06 | $0.10−0.30 | $2.40−7.20 | $10-$30 |
| Procedure time3 | – | ∼1−1.5 hours | 2−3 hours | 3−5 hours |
| Master mix setup (96) | – | 4 min. | 15 min. | 30 min. |
| Sample processing/dilution | – | 15 min. | 60 min. | 90 min. |
| Transfer to Master Mix | – | 5 min. | 15 min. | 30 min. |
| Isothermal amplification | – | 35 min. | 35 min. | 35 min. |
| Evaluate and record result | – | 6 min. | 15 min. | 45 min. |
| Repeat failed reactions | – | 45 min. | 65 min. | 110 min. |
1
Assuming an average of four reactions per screen (multiple dilutions and/or primer sets), plus 20% for cost of positive and negative controls (4 per 48 reactions) and re-tests (6 per 48 reactions).
2
Cost variance due to different sizes of kits and formulations available and whether 20 or 25 μl test volume is used.
3
Does not include sample collection or transport time.