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PCR amplification in mock specimens with PBS or healthy saliva as a solvent.
| RNA (copy/mL) | PBS | Healthy saliva from volunteers A and B |
|---|---|---|
| 5 × 104 | + | +* |
| 1 × 104 | + | – |
| 5 × 103 | + | – |
| 1 × 103 | – | – |
One of the duplicates exhibited an increase of the amplification curve within 40 cycles.
