Table 4.

Spike mutations located within the RBD (AA 330–530) with at least two cases in the population and non-zero GBPM average score in the ACE2/Spike interaction models.

				GBPM
Mutation	Position	Abundance	Frequency	Average score	Quartile
S477N	477	16,547	0.055995	–18.61	Q2
N439K	439	5587	0.018906	–11.81	Q2
N501Y	501	4921	0.016653	–63.3975	Q1
Y453F	453	917	0.003103	–0.8825	Q4
E484K	484	352	0.001191	–5.4375	Q3
K417N	417	260	0.00088	–13.925	Q2
S477I	477	157	0.000531	–18.61	Q2
G446V	446	58	0.000196	–9.6475	Q3
F490S	490	53	0.000179	–18.07	Q2
S477R	477	49	0.000166	–18.61	Q2
N501T	501	47	0.000159	–63.3975	Q1
L455F	455	44	0.000149	–14.3075	Q2
G476S	476	43	0.000146	–18.3675	Q2
E484Q	484	43	0.000146	–5.4375	Q3
A475V	475	35	0.000118	–54.45	Q1
F486L	486	34	0.000115	–42.1525	Q1
F490L	490	18	6.09E-05	–18.07	Q2
YQ505WK	505	14	4.74E-05	–28.835	Q1
Q493L	493	12	4.06E-05	–60.8475	Q1
V503F	503	9	3.05E-05	–4.09	Q3
E484A	484	8	2.71E-05	–5.4375	Q3
G446S	446	7	2.37E-05	–9.6475	Q3
E484D	484	4	1.35E-05	–5.4375	Q3
Q493*	493	4	1.35E-05	–60.8475	Q1
Y505W	505	4	1.35E-05	–28.835	Q1
G476A	476	3	1.02E-05	–18.3675	Q2
S477G	477	3	1.02E-05	–18.61	Q2
F456L	456	2	6.77E-06	–31.21	Q1
V503I	503	2	6.77E-06	–4.09	Q3
Y449F	449	2	6.77E-06	–19.3075	Q1

The asterisk (*) indicates a stop codon. A lower GBPM score indicates a stronger effect in the ACE2/Spike interaction.