Table 2.

Direct enhancement of T cell effector function via CRISPR/Cas9-mediated genome editing.

Species	Target		CAR	In vitro										In vivo					Ref
	Gene	Protein		IFN-γ	TNF-α	IL-2	IL-17	CD107a	GzmA	GzmB	GzmC	GzmD	Cytotoxicity	Model	IFN-γ	IL-2	GzmB	Tumor
Human	DGKA/DGKZ	DGKα/ζ	EGFRvIII	+		+							+	Glioblastoma	+		+	R	[51]
	IFNG 3′UTR	IFN-γ		+	=	=													[48]
	PTPN2	PTPN2	Mesothelin	+	=	=	+												[52]
	TGFBR2	TGFBR2		+		+			+	+			+	Lung cancer	+			R	[56]
Mouse	Dhx37	Dhx37		+				+		+	+	+	+	E0771-OVAMammary tumor				D	[53]
	Gata3	Gata3												B16-F10 melanoma	+	+		D	[50]
	Nr2f6	Nr2f6		+										B16-OVA melanoma				D	[55]
	Zcd3h12a	Regnase-1												B16-OVA melanoma	+	+	+	D	[54]

+, increased production; =, equal production; R, tumor regression; D, tumor outgrowth delayed. Blank indicates not reported.