Table 1.
Technology Platform and Assay Description.
| Platform (Vendor Name) | Simoa (Quanterix) | MESO V-Plex (Mesoscale Discovery) | Luminex xMAP (Myriad) | Luminex (R&D Systems) |
Quantikine (R&D Systems) |
|---|---|---|---|---|---|
| Instrument required | Simoa HD-1 Analyzer and associated assay kits | MESO QuickPlex SQ - Custom | Luminex 100/200™ instrument | Luminex MAGPIX | Quantikine High Sensitivity ELISA Kit |
| Assay format | singleplex; multiplex capabilities | multiplex | multiplex | multiplex | singleplex |
| Assay Descriptions | Automated paramagnetic bead-based ligand binding assay used to concentrate a diluted solution of molecules; enables digital detection of fluorescence product molecules confined to wells of very small volumes (40 fl). | Custom multiplex plates w/ 12 analyte capture deposited on a carbon sensor surface. Utilizes electrochemiluminescence labels for detection of antibodies. | Simultaneously measures multiple biochemical markers; assays are multiplexed into analyte-specific microspheres that are passed single-file through laser beams to attain fluorescent signatures. | Simultaneously measures multiple biochemical markers; assays are multiplexed into analyte-specific microspheres that are passed single-file through laser beam; uses magnetic antibody-coated beads. | Singleplex plate-based ligand binding assays based on a two-site sandwich principle in which two highly specific antibodies detect target analyte, with tyramide-signal amplification and chromogenic detection. |
| Volume required | 220–350 ul/well | 350 ul/well | 100 ul/well | 125 ul/well or 75 ul/well | 50–250 ul/well |