Table 2.
Vendor-provided information on units of measure, dilution factor necessary for assessing the sample on each platform, and lower- and upper-limit of quantification per platform and each cytokine (IL-Iß, IL-6, IFN-γ and TNF-α).
| Analyte | Measurement | Simoa (Quanterix) | MESO V-Plex (Mesoscale Discovery) | Luminex (Myriad) | Luminex (R&D Systems) |
Quantikine (R&D Systems) |
|---|---|---|---|---|---|---|
| IL-1ß | Units | pg/mL | pg/mL | pg/mL | pg/mL | pg/mL |
| Dilution | 4 | 2 | 5 | NA | NA | |
| LLOQ | 0.166 | 2.14 | 5.6 | 0.64 | 0.125 | |
| ULOQ | 48 | 375 | NR | 2600 | 8 | |
| IL-6 | Units | pg/mL | pg/mL | pg/mL | pg/mL | pg/mL |
| Dilution | 4 | 2 | 5 | NA | NA | |
| LLOQ | 0.044 | 1.72 | 4.1 | 1.66 | 0.156 | |
| ULOQ | 94.4 | 531 | NR | 6800 | 10 | |
| IFN-γ | Units | pg/mL | pg/mL | pg/mL | pg/mL | pg/mL |
| Dilution | 4 | 2 | 5 | NA | NA | |
| LLOQ | 0.306 | 8.67 | 5.7 | 8.46 | 15.6 | |
| ULOQ | 800 | 1088 | NR | 34,650 | 1000 | |
| TNF-α | Units | pg/mL | pg/mL | pg/mL | pg/mL | pg/mL |
| Dilution | 4 | 2 | 5 | NA | NA | |
| LLOQ | 0.204 | 0.858 | 62 | 1.52 | 0.156 | |
| ULOQ | 170 | 308 | NR | 6200 | 10 |
Note: NA indicates not applicable, NR indicates not reported.