Table 5.
Statistical parameters of the measured cytokine concentrations, %CV between replicate runs, and the FEAD (BLQ%) across the technology platforms for serum samples derived from the healthy controls*.
| Simoa (Quanterix) | MESO V-Plex (Mesoscale Discovery) | Luminex xMAP (Myriad) |
Luminex (R&D Systems) |
Quantikine (R&D Systems) |
||
|---|---|---|---|---|---|---|
| Cytokine | Measure | HCs | HCs | HCs | HCs | HCs |
| IL-1ß | Median (St Dev) | 0.5 (0.05) | 0.81 (1.51) | 3.23 (0.47) | 0.23 (0.47) | 0.07 (0.02) |
| Min-Max | 0.04 – 0.07 | 33.2–136.2 | 2.9–3.9 | 0–6.15 | 0.02–0.095 | |
| %CV (St Dev) | 25 (10.8) | 85.1 (50.0) | NA | 0.0 (2.9) | 20.6 (15.5) | |
| BLQ % | 40% | 80% | 100% | 100% | 100% | |
| IL-6 | Median (St Dev) | 1.59 (0.80) | 1.0 (0.41) | 2.30 (0.0) | 2.63 (0.70) | 3.03 (4.60) |
| Min-Max | 0.92–3.05 | 0.53–1.65 | 2.3 | 1.27–3.20 | 1.36 (3.58) | |
| %CV (St Dev) | 0.3 (0.1) | 4.5 (4.0) | NA | 0.0 (2.1) | 1.0 (2.1) | |
| BLQ % | 0% | 100% | 100% | 20% | 0% | |
| IFN-γ | Median (St Dev) | 0.18 (0.11) | 2.76 (0.66) | 2.0 (0.0) | NA | 3.2 (2.10) |
| Min-Max | 0.07–0.35 | 3.55 (0.85) | 2 | NA | 0.5–5.6 | |
| %CV (St Dev) | 7.3 (11.2) | 12.4 (39.0) | NA | 2.7 (3.3) | NA | |
| BLQ % | 80% | 100% | 100% | 100% | 100% | |
| TNF-α | Median (St Dev) | 0.95 (0.60) | 1.07 (0.69) | 21.33 (10.66) | 5.94 (3.33) | 0.35 (0.32) |
| Min-Max | 0.91–2.3 | 0.54–2.6 | 11.0–36.0 | 0.8–10.62 | 0.20–1.10 | |
| %CV (St Dev) | 13.9 (3.7) | 2.0 (3.3) | NA | 1.9 (1.6) | 2.1 (6.4) | |
| BLQ % | 0% | 80% | 100% | 20% | 0% |
High: Precision, CV ≤ 20%; FEAD (BLQ%) < 50%.
Intermediate: Precision, CV 20–75%; FEAD (BLQ%) 50–75%.
Low: Precision, CV > 75%; FEAD (BLQ%) > 75.
*
Assay performance was ranked as follows.