Table A2.
Intra-assay precision (%CV) across the technology platforms for paired technical replicates of healthy control samples plated at different positions within a plate.
| Cytokine | Replicate Type | Simoa (Quanterix) | MESO V-Plex (Mesoscale Discovery) | Luminex xMAP (Myriad) | Luminex (R&D Systems) | Quantikine (R&D Systems) |
|---|---|---|---|---|---|---|
| IL-1beta | Plasma Pool 1 | 10.5 | NA | NA | 51.2 | 23.4 |
| Plasma Pool 3 | 3.9 | 111.0 | NA | 8.5 | 57.6 | |
| Healthy Control 5 | 4.8 | 95.7 | NA | 0.0 | 9.9 | |
| IL-6 | Plasma Pool 1 | 4.6 | 2.5 | NA | 7.1 | 0.2 |
| Plasma Pool 3 | 3.8 | 20.3 | NA | 5.3 | 0.9 | |
| Healthy Control 5 | 4.6 | 12.4 | NA | 4.9 | 11.0 | |
| IFN-γ | Plasma Pool 1 | 9.5 | NA | NA | NA | |
| Plasma Pool 3 | 1.2 | 57.9 | 33.3 | NA | NA | |
| Healthy Control 5 | 16.2 | 3.3 | NA | NA | NA | |
| TNF-α | Plasma Pool 1 | 7.4 | 9.0 | NA | 5.4 | 2.1 |
| Plasma Pool 3 | 2.8 | 1.9 | 43.3 | 3.2 | 3.6 | |
| Healthy Control 5 | 14.2 | 2.7 | 0.0 | 1.5 | 1.4 |