Table A3.
Inter-assay precision (%CV) across the technology platforms for paired technical replicates of healthy control samples on plate 1 vs plate 2.
| Cytokine | Replicate Type | Simoa (Quanterix) | MESO V-Plex (Mesoscale Discovery) | Luminex xMAP (Myriad) | Luminex (R&D Systems) | Quantikine (R&D Systems) |
|---|---|---|---|---|---|---|
| IL-1beta | Plasma Pool 1 | 0.1 | 129.3 | NA | 23.6 | 18.1 |
| Plasma Pool 2 | 10.7 | NA | NA | 19.3 | 35.9 | |
| Serum Pool 1 | 23.0 | NA | NA | 12.5 | BLD | |
| IL-6 | Plasma Pool 1 | 1.2 | 8.5 | NA | 3.9 | 1.0 |
| Plasma Pool 2 | 7.0 | 1.8 | NA | 9.0 | 1.0 | |
| Serum Pool 1 | 10.2 | 1.6 | NA | 34.5 | 1.9 | |
| IFN-γ | Plasma Pool 1 | 2.1 | 83.7 | NA | NA | 14.3 |
| Plasma Pool 2 | 7.5 | 53.4 | NA | NA | 75.0 | |
| Serum Pool 1 | 13.3 | 16.4 | 0.0 | NA | NA | |
| TNF-α | Plasma Pool 1 | 0.1 | 6.2 | NA | 2.7 | 2.8 |
| Plasma Pool 2 | 7.5 | 0.8 | NA | 8.4 | 0.4 | |
| Serum Pool 1 | 23.0 | 12.2 | 61.3 | 2.5 | 5.2 |
*Assay Performance was ranked as follows:
High: Precision, CV < 20%.
Intermediate: Precision, CV 20–75%.
Low: Precision, CV > 75%.