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[Preprint]. 2021 Feb 10:2021.02.10.430499. [Version 1] doi: 10.1101/2021.02.10.430499

Figure 2. Bifurcated monocyte activation states in severe COVID-19.

Figure 2.

a) Heatmap displaying hierarchical clustering of Arboreto delineated gene modules that were statistically significantly associated with COVID-19 outcome. Patient samples (day 1 ICU) are arrayed in columns and gene modules in rows. Color bar on the left indicates linkage of gene modules with particular immune cell types. b) Heatmap displaying hierarchical clustering of differentially expressed genes associated with outcome from scRNAseq of peripheral blood monocytes from COVID-19 patients (day 1 ICU). Cells from patient samples with the indicated outcomes are arrayed in columns and genes are annotated in rows. c) Gene set enrichment analysis of canonical monocyte subsets in the scRNAseq dataset. d) Representative flow plots of ACE2 expression on classical and intermediate monocytes. e) Expression levels (mean fluorescence intensity, MFI), on classical and intermediate monocytes in indicated patient groups versus healthy donors. f) Representative flow plots of NRP1 expression on classical and intermediate monocytes. g) Expression levels of NRP1 (MFI) on classical and intermediate monocytes in indicated patient groups versus healthy donors.