Table 1. Kinetic Parameters for Gd11olSWT and Mutants.
| KM (μM) | kcat (10–5 s–1) | kcat/KM (10–5 μM–1 s–1) | |
|---|---|---|---|
| Gd11olSWT | 0.5 ± 0.08 | 52.4 ± 0.02 | 116.4 |
| R228V | 0.8 ± 0.10 | 18.14 ± 0.04 | 22.7 |
| Q313A | 1.10 ± 0.14 | 6.9 ± 0.02 | 6.3 |
| Q313N | 0.5 ± 0.07 | 5.02 ± 0.01 | 10.04 |
| H320A | 1.12 ± 0.10 | 3.8 ± 0.01 | 3.4 |
| H320F | 1.8 ± 0.5 | 2.3 ± 0.02 | 1.3 |
| V187D | N/Aa | ||
| V187N | NDb | ||
| V187A | 0.3 ± 0.06 | 19.4 ± 0.01 | 64.7 |
| G188V | N/Aa | ||
| G188A | 3.8 ± 1.5 | 3.7 ± 0.5 | 1.0 |
| G188S | 5.7 ± 0.9 | 4.08 ± 0.2 | 0.7 |
| A190V | 4.6 ± 2.2 | 1.3 ± 0.3 | 0.3 |
| A190L | N/Aa | ||
| W312F | 0.9 ± 0.09 | 25.5 ± 0.06 | 28.3 |
| W312A | 0.4 ± 0.07 | 4.02 ± 0.01 | 10.05 |
a
Not applicable (inactive).
b
Not determined due to low activity.