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. 2020 Dec 4;9:20. doi: 10.12703/r/9-20

Figure 6. Prime editing and genetic diversification.

Figure 6.

(A) Gene correction by prime editing using Cas9n-RT complexed with the pegRNA. (B) Targeted mutagenesis by converting cytidines or guanines to the other three bases at desired loci using dCas9-AIDx. (C) Continuous diversifying all nucleotides within a tunable window length at a target locus using Cas9n-PolI3M. AID, activation-induced cytidine deaminase; Cas9n, Cas9 nickase; MS2, bacteriophage coat proteins; pegRNA, prime-editing guide RNA; PolI3M, fidelity-reduced variant of Escherichia coli DNA polymerase I (PolI) harboring the mutations D424A, I709N, and A759R; RT, reverse transcription; SNP, single-nucleotide polymorphism.