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. 2021 Jan 25;17(1):e1008761. doi: 10.1371/journal.pgen.1008761

Fig 5. SUTs whose deletion results in global transcriptional changes can rescue growth phenotypes in trans.

Fig 5

Rescue spot test analysis of growth phenotypes of ncRNA deletion mutant strains. The indicated SUT under control of the GAL1 promoter, was spotted at 30°C onto SD + 2% glucose (A), SD + 2% galactose (B), SD + 2% glucose and 5% ethanol (C) and SD + 2% galactose and 5% ethanol (D). Additionally, the spot assay was carried out at 37°C in SD + 2% glucose (E) and SD + 2% galactose (F). +: pRS416 with the respective SUT;—: empty plasmid.