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. 2021 Feb 16;16(2):e0245986. doi: 10.1371/journal.pone.0245986

Fig 2. Sulforaphane suppresses the expression of inflammatory cytokines in the joints of CIA mice.

Fig 2

(A) Immunohistochemical staining for interleukin (IL)-6, IL-17, tumor necrosis factor (TNF)-α, receptor activator of NF-κB ligand (RANKL), and tartrate-resistant acid phosphatase (TRAP) in the synovium of CIA mice. Data are means ± standard deviation (SD) of three independent experiments. (B) Splenocytes from C57BL/6 mice were cultured with sulforaphane (1, 5, 10, or 50 μM) for 72 h, and the IL-6, IL-17, and TNF-α levels in the culture supernatant were measured by ELISA. (C) Cell viability by MTT analysis. Data are means ± SD. ***P < 0.001, **P < 0.01, *P < 0.05 (bars represent means).