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. 2021 Feb 16;16(2):e0245986. doi: 10.1371/journal.pone.0245986

Fig 3. Sulforaphane alters B cell differentiation.

Fig 3

(A) Splenocytes isolated from mice were analyzed by flow cytometry. The cells were stained with antibodies against CD138+B220low B cells for plasma B cells and GL7+B220+ B cells for germinal-center B cells. (B) Splenocytes were cultured with sulforaphane (5 or 10 μM) in the presence of 100 ng/mL lipopolysaccharide (LPS) for 3 days. The population of plasma B cells and germinal-center B cells were analyzed by flow cytometry. (B) Serum level of type II collagen (CII)-specific IgG 45 days after the first immunization. ***P < 0.001, **P < 0.01, *P < 0.05.