Fig 1. Target site and design of new CRISPR^h gene drives designed to express Cas9 under regulation of zpg, nos and exu germline promoters.

(A) The haplosufficient female fertility gene, AGAP007280, and its target site in exon 6 (highlighted in grey), showing the protospacer-adjacent motif (highlighted in teal) and cleavage site (red dashed line). (B) CRISPR^h alleles were inserted at the target in AGAP007280 using φC31-recombinase mediated cassette exchange (RMCE). Each CRISPR^h RMCE vector was designed to contain Cas9 under transcriptional control of the nos, zpg or exu germline promoter and terminator, a gRNA targeted to AGAP007280 under the control of the ubiquitous U6 PolIII promoter, and a 3xP3::DsRed marker.