Table 1. Summary of fecal VirHRV shedding and diarrhea following VirHRV challenge (PCD 1–6).
| HRV Sheddingb | Diarrheac | |||||||
|---|---|---|---|---|---|---|---|---|
| Groupsa | N | Geometric mean of peak titer shed (FFU/ml)d | Mean days to onset of HRV shedding | Mean duration of HRV shedding | Diarrhea (%) | Mean cumulative fecal scoree | Mean days of onset of diarrhea | Mean duration of diarrheaf |
| EcN biofilm | 3 | 97.9* | 1.3 | 2.3 | 0 | 4.0* | N/A | N/A |
| EcN suspension | 4 | 112.0** | 1.0 | 4.0 | 50.0 | 5.5** | 2.5 | 1.3 |
| Control | 3 | 439.7*** | 1.0 | 6.0 | 66.7 | 4.7*** | 3.5 | 1.0 |
a Gnotobiotic (Gn) pigs were fed deficient diet and were transplanted with human infant fecal microbiota (HIFM) at 4 days of age, post-HIFM transplantation day (PTD) 0. Pigs were colonized with a single dose of EcN biofilm and EcN suspension at PTD 11, subsequently challenged with virulent human rotavirus (VirHRV) at PTD 13 and euthanized at PTD27/post challenge day (PCD) 14.
b Determined by cell culture immunofluorescence (CCIF) assay and expressed as FFU/ml.
c Pigs with fecal score > 1 were considered as diarrheic. Fecal consistency was scored as follows: 0, normal; 1, pasty; 2, semi-liquid; and 3, liquid.
d Samples negative for HRV detection (< 25) were assigned a titer of 12.5 for statistical analysis. Means in the same column with different asterisks differ significantly (determined by two-way ANOVA followed by Bonferroni posttest, p < 0.05).
e Mean of total of fecal scores from PCD 1–6.
f Mean of total days with fecal score > 1.
N/A = data not available.