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. Author manuscript; available in PMC: 2021 Feb 16.
Published in final edited form as: Vaccine. 2017 May 5;35(24):3272–3278. doi: 10.1016/j.vaccine.2017.04.047

Fig. 2.

Fig. 2.

Rubella vectors expressing engineered outer domains eOD-GT6 and GT8. (A) HIV-1 envelope glycoprotein gp120 was divided into the inner domain (blue) and outer domain (olive). The engineered outer domain (eOD) sequence had a V3 deletion, and its sequence was permuted to stabilize the native conformation. (B) The eOD insert was attached via flexible linker to an MPERF tag, followed by E2 TM and rubella E1 signal peptide. (C) Expression of minimally (42 kD, red arrow) or fully (58 kD, blue arrow) glycosylated eOD by rubella vectors was detected by western blot with monoclonal 2F5. eOD-GT6 and GT8 were stably expressed through passages P6 and P7. Controls included cells infected with empty rubella virus or AT-2 inactivated SHIV virions expressing gp41 of HIV.