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. Author manuscript; available in PMC: 2021 Feb 16.
Published in final edited form as: Vaccine. 2017 May 5;35(24):3272–3278. doi: 10.1016/j.vaccine.2017.04.047

Fig. 4.

Fig. 4.

Immunoprecipitation (IP) of eOD-GT8 by broadly neutralizing monoclonal NIH45–46 and its germline VH1–2*02 precursor. Cell lysates infected with rubella/eOD-GT8 or with a control rubella/gp120 core protein were studied for total content prior to IP (left panel), after IP with mature monoclonal NIH45–46 (middle panel), or after IP with the germline precursor of VRC01 and NIH45–46 (right panel). The immobilized antibodies captured eOD-GT8 or gp120 core antigen, followed by elution and detection by western blot using monoclonal 2F5 specific for an MPER tag in both constructs. The center panel shows that both antigens bind monoclonal NIH45–46. However, the right panel shows that only eOD-GT8 can bind both the germline precursor of VRC01 and its mature form equally well. IgH and IgL bands correspond to heavy and light chains of the monoclonal antibodies used for IP.