Fig 1. Acentric sister separation is delayed relative to kinetochore-bearing chromosomes during anaphase.

(A) Still frames of a time-lapse movie of a mitotic neuroblast labeled with H2Av-RFP and not expressing I-CreI. (B) Still frames of a time-lapse movie of a mitotic neuroblast with I-CreI induced acentrics. Separation of sister acentrics (arrows) is delayed. Consequently, they lag on the spindle equator but eventually separate, segregate, and are reincorporated into daughter nuclei. Bars, 2 μm. Time in seconds. (C) Scatterplot showing the delay in acentric sister separation after anaphase onset. Delay (seconds) was measured from when kinetochore-bearing chromosomes initiated separation to when acentric sister chromosomes initiated separation. Bars represent mean and standard deviations.