Table 2. Sister separation of acentrics is delayed relative to sister separation of intact chromosomes.

Average time (in seconds) for sister acentrics to initiate separation after sister kinetochore-bearing chromosomes separated. Time (average ± SD) measured from initiation of intact chromosome separation to initiation of acentric sister chromosome separation. Asterisks indicate statistical significance (**P = 0.001, *P = 0.003) as determined by two-sided Mann-Whitney tests.

	Avg ± SD (seconds)	N (acentric pairs)
Control	148 ± 44	19
MAP205 RNAi	97 ± 39 **	12
EB1 RNAi	142 ± 57	16
Topo II RNAi	226 ± 77 *	10
gwl1028/gwl716	138 ± 49	22