. 2021 Feb 16;16(2):e0246992. doi: 10.1371/journal.pone.0246992

Table 2. In vitro ruminal gas production parameters of red grape pomace spent oyster mushroom substrate.

	¹Substrates						Significance
²Parameters	GP0	GP20	GP30	GP40	GP50	³SEM	Linear	Quadratic
a	4.27^a	6.02^b	5.44^ab	6.43^bc	7.80^c	0.425	0.000	0.468
b	18.11^b	15.56^ab	14.54^ab	13.18^a	12.26^a	1.035	0.000	0.922
c (%/h)	0.016^a	0.024^ab	0.029^b	0.028^ab	0.026^ab	0.003	0.018	0.036
PGas	22.38	21.58	19.98	19.62	19.03	1.103	0.037	0.848
EGas	11.85^a	14.43^b	13.91^ab	13.71^ab	13.64^ab	0.562	0.009	0.477

^a,b,c In a row, different superscripts denote significant differences (P < 0.05) between spawning rates.

¹Substrates: GP0 = uninoculated red grape pomace; GP20 = red grape pomace inoculated with oyster mushroom spawn at 200 g/kg; red grape pomace inoculated with oyster mushroom spawn at 300 g/kg; GP40 = red grape pomace inoculated oyster mushroom spawn at 400 g/kg; GP50 = red grape pomace inoculated with oyster mushroom spawn at 500 g/kg.

²Parameters: a = the immediate fermentation fraction; b = the slowly fermentable fraction; c = fermentation rate of fraction (b); PGas = potential gas production; EGas = effective gas production.

³SEM = standard error of the mean.