Table 2.
Efflux Activity at Varying Concentrations of Ethidium Bromide and Corresponding gyrA and parC Gene Mutations
| No | Isolate | Concentration of EtBr + Ciprofloxacin at Which Bacteria Started to Fluoresce (μg/mL) | gyrA | parC | Efflux Activity |
|---|---|---|---|---|---|
| 1. | K1 | 1024 | Ser83Leu, Asp87Asn | Ser80Ile | + |
| 2. | K3 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | + |
| 3. | K6 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | + |
| 4. | K9 | 64 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 5. | K12 | 1024 | Ser83Leu, Asp87Asn | Ser80Ile | + |
| 6. | K13 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 7. | K15 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | + |
| 8. | K17 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | + |
| 9. | K18 | 1024 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 10. | K19 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 11. | K20 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 12. | K21 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 13. | K23 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 14. | K25 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 15. | K26 | 256 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 16. | K27 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 17. | K28 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 18. | K29 | 256 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 19. | K30 | 256 | Ser83Leu, Asp87Asn | Ser80Ile | + |
| 20. | K31 | 512 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 21. | K32 | 64 | Ser83Leu, Asp87Asn | Ser80Ile | – |
| 22. | K33 | 512 | Ser83Leu, Asp87Asn | – | + |
| 23. | K35 | 512 | Ser83Leu, Asp87Asn | – | + |
| 24. | K36 | 512 | Ser83Leu, Asp87Asn | – | – |
| 25. | K41 | 512 | Ser83Leu | – | – |
| 26. | K42 | 512 | Ser83Leu | – | – |
| 27. | K43 | 1024 | Ser83Leu | – | + |