(A) Myenteric iEAN numbers in duodenum, ileum, and colon of C57BL/6J and Casp11−/− mice treated with antibiotics (vancomycin, ampicillin, metronidazole, and neomycin, referred to collectively as Abx) or Splenda. (B and C) Ileal myenteric iEAN numbers for (B) Snap25ΔNlrp6 or (C) Snap25ΔCasp11 mice on Abx. (D) Ileal myenteric iEAN numbers for C57BL/6J SPF mice after 4 weeks on Abx or Splenda, or Abx followed by Splenda for 2 weeks each (Switch). (E) Ileal myenteric iEAN numbers for C57BL/6J SPF mice on metronidazole (Metro), ampicillin (Amp), vancomycin (Vanco), neomycin (Neo), or Splenda. (F) CART+ myenteric iEAN numbers in duodenum, ileum, and colon of C57BL/6J and Casp11−/− mice on Abx or Splenda. (G and H) CART+ ileal myenteric iEAN numbers for (G) Snap25ΔNlrp6 and control or (H) Snap25ΔCasp11 and control mice on Abx. *P < 0.05, **P < 0.01, and ****P < 0.0001; for (A) and (F), two-way ANOVA with Tukey’s multiple comparisons test was performed; for (B), (D), (E), and (G), one-way ANOVA with Tukey’s multiple comparisons test was performed. Error bars indicate SD.