Skip to main content
. Author manuscript; available in PMC: 2021 Apr 16.
Published in final edited form as: Science. 2020 Aug 27;370(6514):314–321. doi: 10.1126/science.abd6176

Fig. 4. CART+ iEANs in the distal intestine are viscerofugal and glucoregulatory.

Fig. 4.

(A and B) Whole-mount IF image of the (A) ileum myenteric plexus (MP) and (B) CG-SMG of CartCre+ injected with AAVrg-FLEX-tdTomato into (A) ileum and (B) duodenum, ileum, and colon. (C and D) Whole-mount IF image of the ileum MP of CartCre+ mice injected with AAV9-hSyn-DIO-hM3Dq-mCherry into the ileum, (C) stained for CART (green) and mCherry (red) and (D) 3 hours after C21 administration, stained for mCherry (red) and cFos (green). (E) Food consumption at night 2 hours (left) and 4 hours (right) after C21 administration in CartCre+ and CartCre− mice injected with AAV9 as in (C). (F) Blood glucose levels after C21 administration (left) and area under curve (AUC) analysis (right) in fed CartCre+ and CartCre− (control) mice injected with AAV9-hSyn-DIO-hM3DqmCherry or control AAV9-hSyn-DIO-mCherry into ileum and colon. (G and H) Plasma (G) insulin and (H) glucagon levels after C21 administration in CartCre+ and CartCre− mice injected with AAV9-hSyn-DIO-hM3Dq-mCherry into ileumand colon. Scale bars in (A) to (D) are 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; for (E) and (F), two-tailed unpaired Student’s t test was performed; for (G), two-way (left) or one-way ANOVA (right) with Tukey’s multiple comparisons test was performed. Error bars indicate SD.