Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. musculus) | Olig3CreERT2 | Storm et al., 2009. | RRID:MGI:3833734 | |
| Strain, strain background (M. musculus) | Olig3GFP | Müller et al., 2005. | ||
| Strain, strain background (M. musculus) | MaptnLacZ | Hippenmeyer et al., 2005. | The Jackson Laboratory, Stock No: 021162 | |
| Strain, strain background (M. musculus) | Rosa26lsl-tdT (Ai14) | Madisen et al., 2010. | The Jackson Laboratory, Stock No. 007908 | |
| Strain, strain background (M. musculus) | Olig2Cre | Dessaud et al., 2007. | ||
| Antibody | Anti-β-gal (Chicken polyclonal) |
Abcam | ab9361 RRID:AB_307210 |
(1:1,000) |
| Antibody | Anti-GFP (Chicken polyclonal) |
Abcam | ab13970 RRID:AB_300798 |
(1:500) |
| Antibody | Anti-Brn2 (Goat polyclonal) |
Abcam | ab101726 RRID:AB_10710183 |
(1:1,000) |
| Antibody | Anti-Foxp2 (Goat polyclonal) |
Abcam | ab58599 RRID:AB_941649 |
(1:1,000) |
| Antibody | Anti-Olig3 (Guinea pig polyclonal) |
Gift from T. Muller | Homemade | (1:5,000) |
| Antibody | Anti-Foxp2 (Rabbit polyclonal) |
Abcam | ab16046 RRID:AB_2107107 |
(1:1,000) |
| Antibody | Anti-GFP (Rabbit polyclonal) |
Abcam | ab290 RRID:AB_303395 |
(1:500) |
| Antibody | Anti-Pax2 (Rabbit monoclonal) |
Abcam | EP3251 RRID:AB_1603338 |
(1:1,000) |
| Antibody | Anti-Sox2 (Rabbit polyclonal) |
Abcam | ab97959 RRID:AB_2341193 |
(1:1,000) |
| Antibody | Anti-Tbr1 (Rabbit polyclonal) |
Abcam | ab31940 RRID:AB_2200219 |
(1:1,000) |
| Antibody | Anti-Tbr2 (Rabbit polyclonal) |
Abcam | ab23345 RRID:AB_778267 |
(1:1,000) |
| Antibody | Anti-Ptf1a (Rabbit polyclonal) |
Gift from J. Johnson | Homemade | (1:5,000) |
| Antibody | Anti-Olig2 (Rabbit polyclonal) |
Merck Millipore | AB9610 RRID:AB_570666 |
(1:1,000) |
| Antibody | Anti-Pax6 (Rabbit polyclonal) |
Merck Millipore | AB2237 RRID:AB_1587367 |
(1:1,000) |
| Antibody | Anti-RFP (Rabbit polyclonal) |
Rockland | 600-401-379 RRID:AB_2209751 |
(1:500) |
| Antibody | Anti-Caspase-3 (Rabbit polyclonal) |
R and D Systems | AF835 RRID:AB_2243952 |
(1:1,000) |
| Antibody | Anti-Parvalbumin (Rabbit polyclonal) |
Swant | PV 27 RRID:AB_2631173 |
(1:3,000) |
| Antibody | Anti-Atoh1 (Rabbit polyclonal) |
Gift from T. Jessell | Homemade | (1:10,000) |
| Antibody | Anti-BrdU (Rat monoclonal) |
Abcam | ab6326 RRID:AB_305426 |
(1:2,000) |
| Antibody | Anti-GFP (Rat monoclonal) |
Nacalai Tesque | GF090R RRID:AB_10013361 |
(1:2,000) |
| Antibody | Donkey anti-species Alexa Fluor488/568/647 | Jackson ImmunoResearch | Various | (1:500) |
| Commercial Assay or kit | BrdU | Sigma-Aldrich | B5002-1G | 16 mg/ml in 0.9% saline solution |
| Recombinant DNA reagent | pCAG-GFP | Addgene | Plasmid #11150 RRID:Addgene_11150 |
|
| Recombinant DNA reagent | pCAG-Empty-IRES-GFP | This paper | ||
| Recombinant DNA reagent | pCAG-Olig3-IRES-GFP | This paper | vector: pCAG; cDNA fragment: mouse Olig3 | |
| Recombinant DNA reagent | pCAG-Pax2-IRES-GFP | This paper | vector: pCAG; cDNA fragment: mouse Pax2 | |
| Sequence-based reagent (M. musculus) | Mouse Olig3 forward primer | Olig3FW | PCR Primer | ATGAATTCTGATTCGAGC |
| Sequence-based reagent (M. musculus) | Mouse Olig3 reverse primer | Olig3RV | PCR Primer | TTAAACCTTATCGTCGTC |
| Sequence-based reagent (M. musculus) | Mouse Pax2 forward primer | Pax2FW | PCR Primer | ATGGATATGCACTGCAAAGCAG |
| Sequence-based reagent (M. musculus) | Mouse Pax2 reverse primer | Pax2RV | PCR Primer | GTGGCGGTCATAGGCAGC |
| Software, algorithm | GraphPad Prism | GraphPad Software | RRID:SCR_002798 | Prism 8 |
| Software, algorithm | Adobe Photoshop | Adobe | RRID:SCR_014199 | Adobe Photoshop CS6 |
| Software, algorithm | ImageJ | NIH | RRID:SCR_002285 | |
| Software, algorithm | Arivis Vision4D | Arivis | RRID:SCR_018000 | Arivis Vision4D 3.2 |