Table 2. Description of the statistical analyses used in this study.
| Fig. | N | Descriptive statistics | Test used | p Value | Degrees of freedom and F/t/z/R/ETC |
Pos hoc analysis | Adjusted p value |
|---|---|---|---|---|---|---|---|
| 2E | Three mice (E10.5) three mice (E11.5) three mice (E12.5) three mice (E13.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 67.20 F(DFn, DFd): 0.1441 (3, 8) |
Tukey’s multiple comparative test | As indicated in the figure |
| 2F | Three mice (E10.5) three mice (E11.5) three mice (E12.5) three mice (E13.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 93.57 F(DFn, DFd): 1.868 (3, 8) |
Tukey’s multiple comparative test | As indicated in the figure |
| 2G | Three mice (E10.5) three mice (E11.5) three mice (E12.5) three mice (E13.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 122.2 F(DFn, DFd): 1.096 (3, 8) |
Tukey’s multiple comparative test | As indicated in the figure |
| 2H | Three mice (E10.5) three mice (E11.5) three mice (E12.5) three mice (E13.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 13.65 F(DFn, DFd): 0.8851 (3, 8) |
Tukey’s multiple comparative test | As indicated in the figure |
| 3A | Four control mice four mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
<0.0001 | t = 15.13; df = 6 | - | - |
| 3B | Four control mice four mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
0.0005 | t = 6.742; df = 6 | - | - |
| 3C | Three control mice three mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
0.0005 | t = 10.47; df = 4 | - | - |
| 3D | Three control mice three mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
0.0002 | t = 13.45; df = 4 | - | - |
| 3E | Four control mice four mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
<0.0001 | t = 16.89; df = 6 | - | - |
| 3F | Four control mice four mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
<0.0001 | t = 11.26; df = 6 | - | - |
| 4D | Four control (E13.5) four mutant (E13.5) four control (E14.5) four mutant (E14.5) four control (P0) four mutant (P0) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 498.6 F(DFn, DFd): 1.252 (5, 18) |
Tukey’s multiple comparative test | As indicated in the figure |
| 4E | Four control (E13.5) four mutant (E13.5) four control (E14.5) four mutant (E14.5) four control (P0) four mutant (P0) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 514.2 F(DFn, DFd): 7.873 (5, 18) |
Tukey’s multiple comparative test | As indicated in the figure |
| 4F | Four control mice three mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
<0.0001 | t = 22.92; df = 5 | - | - |
| 5B | Three control-OE mice 7 Pax2-OE mice |
Mean and SD | Unpaired t-test (two-tailed) |
<0.0001 | t = 67.67; df = 8 | - | - |
| 5C | Five control-OE mice 5 Olig3-OE mice |
Mean and SD | Unpaired t-test (two-tailed) |
0.0001 | t = 13.24; df = 8 | - | - |
| 6C | Three control mice three mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
0.8969 | t = 0.1380; df = 4 | - | - |
| 6D | Three control mice three mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
0.6835 | t = 0.4387; df = 4 | - | - |
| 6F | Four control 4 Olig3 mutant 4 Olig2 mutant |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 1416 F(DFn, DFd): 0.8767 (2, 11) |
Tukey’s multiple comparative test | As indicated in the figure |
| 2-fs 1C |
Three mice (E10.5) three mice (E11.5) three mice (E12.5) three mice (E13.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 299.8 F(DFn, DFd): 0.3133 (3, 8) |
Tukey’s multiple comparative test | As indicated in the figure |
| 2-fs 1D |
Three mice (E10.5) three mice (E11.5) three mice (E12.5) three mice (E13.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 100.9 F(DFn, DFd): 0.2601 (3, 8) |
Tukey’s multiple comparative test | As indicated in the figure |
| 2-fs 1E |
Three mice (E10.5) three mice (E11.5) three mice (E12.5) three mice (E13.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 85.64 F(DFn, DFd): 0.08333 (3, 8) |
Tukey’s multiple comparative test | As indicated in the figure |
| 3-fs 1B |
Three control mice three mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
0.0093 | t = 4.707; df = 4 | - | - |
| 3-fs 1C |
Three control mice three mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
0.2799 | t = 1.249; df = 4 | - | - |
| 3-fs 2A |
Three control mice three mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
<0.0001 | t = 16.84; df = 4 | - | - |
| 3-fs 2B |
Three control mice three mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
<0.0001 | t = 17.51; df = 4 | - | - |
| 3-fs 2C |
Three control mice three mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
0.0801 | t = 2.331; df = 4 | - | - |
| 3-fs 3A |
Four control (E11.5) four mutant (E11.5) four control (E12.5) four mutant (E12.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 139.7 F(DFn, DFd): 2.328 (3, 12) |
Tukey’s multiple comparative test | As indicated in the figure |
| 3-fs 3B |
Three control (E11.5) four mutant (E11.5) four control (E12.5) four mutant (E12.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 118.9 F(DFn, DFd): 0.3083 (3, 11) |
Tukey’s multiple comparative test | As indicated in the figure |
| 3-fs 3C |
Three control (E11.5) four mutant (E11.5) three control (E12.5) four mutant (E12.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 232.2 F(DFn, DFd): 0.4627 (3, 10) |
Tukey’s multiple comparative test | As indicated in the figure |
| 3-fs 3D |
Four control (E11.5) four mutant (E11.5) four control (E12.5) four mutant (E12.5) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 156.3 F(DFn, DFd): 0.9549 (3, 12) |
Tukey’s multiple comparative test | As indicated in the figure |
| 3-fs 3E |
Four control (E11.5) four mutant (E11.5) four control (E12.5) four mutant (E12.5) |
Mean and SD | Ordinary one-way ANOVA | 0.1739 | F: 1.960 F(DFn, DFd): 0.4100 (3, 12) |
Tukey’s multiple comparative test | As indicated in the figure |
| 4-fs 2B |
Four control (E13.5) four mutant (E13.5) four control (E14.5) four mutant (E14.5) four control (P0) four mutant (P0) |
Mean and SD | Ordinary one-way ANOVA | <0.0001 | F: 850.2 F(DFn, DFd): 3.630 (5, 18) |
Tukey’s multiple comparative test | As indicated in the figure |
| 5-fs 1B |
Three control-OE mice 3 Pax2-OE mice |
Mean and SD | Unpaired t-test (two-tailed) |
<0.0001 | t = 22.79; df = 4 | - | - |
| 6-fs 1A |
Three control mice four mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
<0.0001 | t = 12.82; df = 5 | - | - |
| 6-fs 1B |
Three control mice four mutant mice |
Mean and SD | Unpaired t-test (two-tailed) |
0.0002 | t = 10.1; df = 5 | - | - |
OE, overexpression; fs, figure supplement.