| Recombinant DNA reagent |
phox2bb |
Uribe and Bronner, 2015 |
|
|
| Recombinant DNA reagent |
sox10 |
Dutton et al., 2001 |
|
|
| Recombinant DNA reagent |
mmp2 |
Mammalian Gene Collection Program Team et al., 2002 |
|
|
| Sequence-based reagent |
notch1a |
Uribe Lab |
|
Forward 5'-CAGTGGACTCAGCAGCATC-3' Reverse 5'-CCTTCCGACCAATCAGACAAG-3' |
| Sequence-based reagent |
dla |
Uribe Lab |
|
Forward 5'-CAGCCAAGTTGCTCAGAG-3' Reverse 5'-GTACAGAGAACCAGCTCATC-3' |
| Sequence-based reagent |
foxc1a |
Uribe Lab |
|
Forward 5'-ATACGGTGGACTCTGTGG-3' Reverse 5'-CAGCGTCTGTCAGTATCG-3' |
| Genetic reagent (Danio rerio) |
AB |
ZIRC |
|
Wild-type zebrafish |
| Genetic reagent (Danio rerio) |
Tg(−4.9sox10:egfp)ba2Tg |
Carney et al., 2006 |
|
GFP Labeled Neural Crest Cells |
| Commercial assay, kit |
mitfa |
Molecular Instruments |
NM_130923.2 |
|
| Commercial assay, kit |
tfec |
Molecular Instruments |
NM_001030105.2 |
|
| Commercial assay, kit |
xdh |
Molecular Instruments |
XM_683891.7 |
|
| Commercial assay, kit |
phox2bb |
Molecular Instruments |
NM_001014818.1 |
|
| Commercial assay, kit |
ngfrb |
Molecular Instruments |
NM_001198660.1 |
|
| Commercial assay, kit |
gfra1a |
Molecular Instruments |
NM_131730.1 |
|
| Commercial assay, kit |
crestin |
Molecular Instruments |
AF195881.1 |
|
| Commercial assay, kit |
foxd3 |
Molecular Instruments |
NM_131290.2 |
|
| Commercial assay, kit |
vipb |
Molecular Instruments |
NM_001114555.1 |
|
| Commercial assay, kit |
elavl3 |
Molecular Instruments |
NM_131449 |
|
| Commercial assay, kit |
oprl1 |
Molecular Instruments |
NM_205589.2 |
|
| Commercial assay, kit |
barx1 |
Molecular Instruments |
NM_001024949.1 |
|
| Commercial assay, kit |
pbx3b |
Molecular Instruments |
BC131865.1 |
|
| Commercial assay, kit |
prrx1b |
Molecular Instruments |
NM_200050.1 |
|
| Commercial assay, kit |
slc18a3a |
Molecular Instruments |
NM_0010775550.1 |
|
| Commercial assay, kit |
hoxb5a |
Molecular Instruments |
NM_131101.2 |
|
| Commercial assay, kit |
hoxb5b |
Molecular Instruments |
bc078285.1 |
|
| Commercial assay, kit |
hoxd4a |
Molecular Instruments |
NM_001126445 |
|
| Commercial assay, kit |
hoxa5a |
Molecular Instruments |
NM_131540.1 |
|
| Commercial assay, kit |
twist1a |
Molecular Instruments |
NM_130984.2 |
|
| Commercial assay, kit |
Single Cell 3' v2 Chemistry Kit for 10,000 cells |
10x Genomics |
CG00052 |
https://support.10xgenomics.com/single-cell-gene-expression/library-prep/doc/user-guide-chromium-single-cell-3-reagent-kits-user-guide-v2-chemistry |
| Commercial assay, kit |
NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) |
illumina |
20024904 |
https://www.illumina.com/products/by-type/sequencing-kits/cluster-gen-sequencing-reagents/nextseq-series-kits-v2-5.html |
| Chemical compound, drug |
1-phenyl 2-thiourea (PTU)/E3 solution |
Karlsson 741 et al., 2001 |
Sigma-Aldrich |
P7629 |
| Chemical compound, drug |
Tricaine |
A5040 |
Sigma |
|
| Chemical compound, drug |
Accumax buffer |
A7089 |
Sigma-Aldrich |
|
| Chemical compound, drug |
Hank’s Buffer |
55021C |
Sigma-Aldrich |
|
| Chemical compound, drug |
Phusion-HF |
M0530S |
New England Biolabs |
|
| Chemical compound, drug |
Zero Blunt TOPO PCR Cloning Kit |
451245 |
Invitrogen |
|
| Software, algorithm |
R v3.6.3 |
R-project |
RRID:SCR_001905
|
https://www.r-project.org/ |
| Software, algorithm |
Seurat v3.1.1 |
Satija Lab |
RRID:SCR_007322
|
https://github.com/satijalab/seurat |
| Software, algorithm |
Fiji |
PMID:22743772
|
RRID:SCR_002285
|
https://imagej.net/Fiji |
| Software, algorithm |
IMARIS v9.2 |
Bitplane |
RRID:SCR_007370
|
Bitplane.com |
| Software, algorithm |
PANTHER |
GENEONTOLOGY Unifying Biology |
RRID:SCR_004869
|
http://pantherdb.org |
| Software, algorithm |
Cell Ranger v2.1.0 |
10x Genomics |
RRID:SCR_017344
|
Zheng et al., 2017, https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger
|
