Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-human CD45-PerCP (Mouse IgG1, κ) RRID:AB_2566358 | BioLegend | Cat# 368506 Clone: 2D1 |
‘(1:20)’ |
| Antibody | Anti- human CD3-FITC (Mouse IgG1, κ) RRID:AB_2562046 | BioLegend | Cat# 399430 Clone: UCHT1 |
‘(1:25)’ |
| Antibody | Anti-human CD4-APC-eF780 (Mouse IgG1, κ) RRID:AB_1272044 | eBioscience | Cat# 47-0049-42 Clone: RPA-T4 |
‘(1:50)’ |
| Antibody | Anti-human CD8-V500 (Mouse IgG1, κ) RRID:AB_2870326 | BD Biosciences | Cat# 561617 Clone: SK1 |
‘(1:60)’ |
| Antibody | Anti-human CD19-eFluor450 (Mouse IgG1, κ) RRID:AB_1272053 | eBioscience | Cat# 48-0199-42 Clone: HIB19 |
‘(1:25)’ |
| Antibody | Anti-human CD45RO-PE-Cy7 (Mouse IgG2A, κ) RRID:AB_647426 | BD Biosciences | Cat# 337168 Clone: UCHL1 |
‘(1:60)’ |
| Antibody | Anti-human CD27-APC (Mouse IgG1, κ) RRID:AB_469371 | eBioscience | Cat# 17-0279-42 Clone: O343 |
‘(1:25)’ |
| Antibody | Anti-human CD31-PE (Mouse IgG1, κ) RRID:AB_400016 |
BD Biosciences | Cat# 340297 Clone: L133.1 |
‘(1:12.5)’ |
| Antibody | Anti-human CD3-eFluor450 (Mouse IgG2A, κ) RRID:AB_1272055 | eBioscience | Cat# 48-0037-42 Clone: OKT3 |
‘(1:50)’ |
| Antibody | Anti-human CD95-PE (Mouse IgG1, κ) RRID:AB_396027 |
BD Biosciences | Cat# 555674 Clone: DX2 |
‘(1:50)’ |
| Antibody | Anti-human CD19-PerCP (Mouse IgG1, κ) RRID:AB_2868816 | BD Biosciences | Cat# 363014 Clone:SJ25C1 |
‘(1:50)’ |
| Antibody | Anti-human IgM-PE (Goat IgG) RRID:AB_2795614 |
Southern Biotech | Cat# 2022–09 Polyclonal |
‘(1:100)’ |
| Antibody | Anti-human Ki-67-FITC (Mouse IgG1, κ) RRID:AB_578716 | DAKO | Cat# F7268 Clone:MIB-1 |
‘(1:10)’ |
| Commercial assay or kit | Cytofix/Cytoperm | BD Biosciences | Cat#554714 | |
| Commercial assay or kit | FACS Lysing Solution | BD Biosciences | Cat#349202 | |
| Commercial assay or kit | Reliaprep Blood gDNA Miniprep System | Promega | Cat#A5081 | |
| Commercial assay or kit | Data acquisition | Luminex | xPONENT software version 4.2 | |
| Commercial assay or kit | Data acquisition | Biorad Laboratories | Biorad FlexMAP3D | |
| Chemical compound | 2H2O, 99.8% enriched | Cambridge Isotope Laboratories | Cat#DLM-2259–1 | |
| Software, algorithm | Data analysis | Biorad laboratories | Biorad Bio-Plex Manager software, version 6.1.1 | |
| Software, algorithm | Data analysis | Biorad laboratories | Biorad FlexMAP3D | |
| Software, algorithm | Data analysis |
Westera et al., 2013
DOI: 10.1182/blood-2013-03-488411 |
Multiexponential model | |
| Software, algorithm | Data analysis | GraphPad PRISM | GraphPad Software |