Table 5.

Phytochemical Screening of 80% Methanol Extract of P. dodecandra Root

Phytochemical	Test Method	Observation	Result
Alkaloids	Bouchardat’s reagent test	Brown precipitate was not observed	Absence of alkaloids
	Dragendorff’s reagent test	Red-orange precipitate was not formed	Absence of alkaloids
	Mayer’s reagent test	Yellowish-white precipitate was not formed	Absence of alkaloids
	Wagner’s reagent test	A red-brown precipitate was not formed	Absence of alkaloids
Anthraquinone Glycosides	Borntrager’s test	Red color was not occurred	Absence of anthraquinone glycosides
	Ammonium hydroxide test	Pink-red color was not formed	Absence of anthraquinone glycosides
Cardiac Glycosides	Keller-Kiliani test	No formation of brown-ring between layers	Absence of cardiac glycosides
Flavonoids	Alkaline reagent test	Yellow fluorescence was not present	Absence of flavonoids
	Shinoda test	Red-pink color was not formed	Absence of flavonoids
Polyphenols	Lead acetate test	A bulky white precipitate was formed	Presence of polyphenols
	Ferric chloride test	Blue-green color was formed	Presence of polyphenols
Saponins	Frothing test	A stable persistent froth was formed	Presence of saponins
Steroids	Libermann-Buchard test	No formation of brown ring at the interface	Absence of steroids
Tannins	Ferric chloride test	Blue or purple or green precipitate was formed	Presence of tannins
Terpenoids	Salkowski test	A reddish-brown precipitate was formed	Presence of terpenoids