Table 4.

Comparison of cell phenotype and cytokine before and after the stimulation of CD4+T cell with candidate epitope peptide.

Group	Control	P39	P62	P39+P62
CD4+T%	36.40 ± 0.91	38.17 ± 1.00∗∗∗	41.96 ± 1.66∗∗∗	44.57 ± 1.18∗∗∗
IFN-γ(pg/mL)	52.29 ± 7.52	86.24 ± 4.91∗∗∗	91.54 ± 1.86∗∗∗	102.72 ± 5.19∗∗∗
TNF-α(pg/mL)	64.42 ± 9.44	83.65 ± 7.30∗∗∗	88.24 ± 5.22∗∗∗	99.53 ± 4.47∗∗∗
IL-2 (pg/mL)	2.96 ± 0.14	4.94 ± 0.27∗∗∗	5.03 ± 0.36∗∗∗	6.11 ± 0.67∗∗∗
IL-10 (pg/mL)	9.91 ± 0.68	9.76 ± 0.53	8.98 ± 0.78	5.87 ± 0.29∗∗∗

According to the normality and homogeneity of variance, the experimental data were analyzed by single factor and multilevel variance analysis, and the Student–Newman–KeμLs test was used for pairwise analysis and comparison of data between groups. When P < .05, there was a significant difference.

^∗P < .05 vs Control group.

^∗∗P < .01 vs Control group.

^∗∗∗P < .001 vs Control group.