Figure 1.
The effect of RSV on SIRT1 activation; FOXO1-SIRT1 interaction; and FOXO1, acetyl-FOXO1, and SIRT1 expression in SAP. Western blot analysis was employed on the expression of SIRT1, FOXO1, and acetyl-FOXO1. (a) Representative results for Western blot analysis of SIRT1, FOXO1, and acetyl-FOXO1 in the pancreases. (b) Semiquantitative analysis of 10 animals studied in each group. The relative amounts of SIRT1, FOXO1, and acetyl-FOXO1 in each group of rats were normalized by β-actin (a). Bars represent the means ± SE (n = 10). ∗∗∗P < 0.05 (SAP vs. sham); #P < 0.05 (RSV vs. SAP); ^P < 0.05 (EX vs. RSV). (c) Immunoprecipitation was employed in the interaction of SIRT1 and FOXO1. (d) The activity of SIRT1.