Figure 3.

Effect of ACE2 N-glycans on RBD-ACE2 interaction. (A) Deglycosylation (dgACE2) was performed by treatment with PNGase F for 1 h at 37°C. Deglycosylation was confirmed via SDS-PAGE stained with EZBlue. (B) The dynamic force spectra of the RBD-dgACE2 interactions are shown. Solid lines are linear fits to Eq. 3 for the interactions. The dashed line is the linear fits for N-glycosylated ACE2 interactions taken from Fig. 1. The bars denote half bin-widths of the unbinding force histograms (see Fig. S4), representing the errors of force determination. Individual data points of RBD^CoV2-dgACE2 (N = 346) and RBD^CoV1-dgACE2 (N = 260) unbinding forces were plotted as scatter plots, using smaller symbols and the same color scheme. To see this figure in color, go online.