Figure 2.

Time course of FNDC5/irisin expression after SAH. (A) Representative western blotting images and relative density analysis of FNDC5/irisin at 6, 12, 24, 48, and 72 h after SAH. Endogenous FNDC5/irisin protein decreased at 6 h while it increased at 12 h and decreased again from 24 to 72 h after SAH. Data were represented as mean ± SD, n = 6 per group; the one-way ANOVA was used followed by the Tukey's HSD post-hoc test and the Holm–Bonferroni correction method. *P < 0.05, **P < 0.01, ***P < 0.001 vs. sham group. (B) FNDC5/irisin could be co-localized with neurons, and the number of FNDC5/irisin-positive neurons was decreased at 24 h after SAH. Scale bar = 50 μm, n = 3 per group; FNDC5, fibronectin domain-containing protein 5; SAH, subarachnoid hemorrhage.