Table 2.

Akt1 phospho-form relative selectivity values.

Gene	Protein name	Peptide sequence	pI	Phospho-form relative selectivity value (x-fold)
				pAkt1 S473	pAkt1 T308	ppAkt1 T308,S473
ZNF256	Zinc finger protein 256	THQRVHTGTRPYM	10.83	6.7	0.3	0.3
CMTM4	MARVEL transmembrane 4	EIQRLDTFSYSTN	4.37	8.3	0.2	0.2
FMO2	Dimethylaniline monooxygenase	FHTRFRSMLRNVL	12.3	4.4	0.2	0.5
KIAA1109	Uncharacterized protein	TSKRALSTWGPVP	11	2.9	0.5	0.6
TRAPPC1	Trafficking protein particle complex 1	FRSRLDSYVRSLP	10.74	3.6	0.3	0.6
BTBD11	Ankyrin repeat and BTB/POZ	MHSRHNSFDTVNT	6.69	2.5	0.4	0.8
SCN2A	Sodium channel protein type 2 alpha	RKRRSSSYHVSMD	10.9	2.1	0.3	1.0
LPHN3	Adhesion G protein-coupled receptor L3	YSKRTMTGYWSTQ	9.7	1.4	0.6	1.0
CDCA7L	Cell division cycle-associated 7-like	RRHRISSFRPVED	11.52	1.7	0.4	1.3
HRH1	Histamine H1 receptor	TWKRLRSHSRQYV	11.72	1.0	0.6	1.5
PITPNM2	Phosphatidylinositol transfer protein 2	PRKRSDSSTYELD	6.54	0.8	0.8	1.5
KCNH2	Potassium channel H2	QRKRKLSFRRRTD	12.18	1.1	0.4	2.0
SRRM4	Serine/arginine repetitive matrix protein 4	KRRRSSSYSPSPV	11.72	0.8	0.5	2.1
CYSLTR1	Cysteinyl leukotriene receptor 1	FRKRLSTFRKHSL	12.31	0.5	0.7	2.3
RAB11FIP2	Rab11 family-interacting protein 2	PHRRTLSFDTSKM	10.84	0.6	0.7	2.3
GRAMD1C	GRAM domain containing 1C	LRRRRRTFNRTAE	12.3	0.8	0.4	2.4

Relative selectivity values of each Akt1 phospho-form are presented for each of the peptides listed. Relative selectivity values were derived from the normalized k_app values of each Akt1 phospho-form for each of the peptides listed (See Results).