FIGURE 3.

Human primary PTEC cultured under inflammatory conditions produce increased numbers of sEV – defined by CD63/CD9/CD81 content. (a) Western Blot for calnexin, CD63, CD9, and CD81 in PTEC lysate (2 μg total protein) and purified apical and basolateral sEV lysates (particles from equivalent 1 cm² area of confluent PTEC monolayer) produced by human primary PTEC under normal and inflammatory (IFN) culture conditions. Representative data from eight individual PTEC donor experiments for calnexin, CD63 and CD9 and three individual PTEC donor experiments for CD81 are presented. (b‐d) Overall peak area signal for CD63 (b), CD9 (c), and CD81 (d) from purified apical and basolateral sEV lysates (particles from equivalent 1 cm² area of confluent PTEC monolayer) produced by human primary PTEC under normal and inflammatory (IFN) culture conditions. Results represent mean ± SEM of eight individual PTEC donor experiments for CD63 and CD9 and three individual PTEC donor experiments for CD81. *P < 0.05, **P < 0.01, Wilcoxon matched‐pairs signed‐rank test.