Table 1.
Summary of the biosynthesis of natural products from xylose using yeast strains.
| Chassis | Engineering strategy | Product | Titer (mg/L) | References |
|---|---|---|---|---|
| Scheffersomyces stipitis | ↑DAHP synthase variant (aro4K220L), ↑ aro1(D900A), and ↑ Tkt1 | Shikimate | 3,110 | Gao et al., 2017 |
| Saccharomyces cerevisiae | ↑ SsXYL1, ↑SsXYL2, ↑SsXYL3, ΔARO10, ΔPDC5, ↑shikimate kinase II (aroL) from E. coli, ↑tyrosine ammonia-lyase (TAL) from Flavobacterium johnsoniae, ↑DAHP synthase mutant, ↑chorismate mutase mutant | p-coumaric acid | 242 | Borja et al., 2019 |
| Saccharomyces cerevisiae | ↑ SsXYL1(K271N), ↑ SsXY2, ↑XKS,↑Gal2(N376F), ↑xPk,↑PTA, ↑tHMG1, ↑CrtEBI, Δpho13, and ΔAld6 | β-Carotene | 903 | Su et al., 2020 |
| Saccharomyces cerevisiae | ↑ SsXYL1, ↑SsXYL2, ↑SsXYL3, ↑TKL1, ↑NpR5600, ↑NpR5599, ↑NpR5598, ↑NpR5597, and ΔTAL1 | Shinorine | 31 | Park et al., 2019 |
| Saccharomyces cerevisiae | ↑ SsXYL1, ↑SsXYL2, ↑SsXYL3, ↑TKL1, ↑ERG10, ↑tHMG1, ↑TAL1, ΔTAL1, Δpho13, and ΔAld6 | Squalene | 532 | Kwak et al., 2017 |
| Saccharomyces cerevisiae | ↑ SsXYL1, ↑SsXYL2, ↑SsXYL3, ↑TKL1, ↑ERG10, ↑tHMG1, ↑TAL1, ↑ADS, ΔTAL1, Δpho13, and ΔAld6 | Amorphadinene | 254 | Kwak et al., 2017 |
| Saccharomyces cerevisiae | ↑ SsXYL1, ↑SsXYL2, ↑SsXYL3, ↑CrtE/I/YB, ↑Blh, Δpho13, and ΔAld6 | Vitamin A | 3,350 | Sun et al., 2019 |
| Yarrowia lipolytica | ↑ XYL1, ↑XYL2, ↑XKS, ↑TAL (tyrosine ammonia lyase), ↑4CL, ↑CHS, ↑CHI, and ↑XT (xylose transporter, YALI0B00396) | Naringenin | 715 | Wei et al., 2020b |