TABLE 4.
Hybrid assembled Escherichia coli food isolates and corresponding beta-lactamase harboring plasmids for comparative analysis.
| Isolate | ESBL-plasmid | Plasmid replicona | pMLST/RST | Plasmid size (bp) | Beta-lactamase gene | Other resistance genes on same plasmid replicon(s) | Virulence genes on plasmid replicon(s) |
| A40.2-1 | pZPK-A40.2-1 | IncFIB, IncFIC(FII) | [F-:A-:B1] FIC-4 | 120698 | blaCTX–M–55, blaTEM–1B | ARR-2, floR, dfrA14, aph(3′′)-Ib, aph(3′)-Ia, aph(6)-Id, tet(A), sul2 | cma, cvaC, hlyF, iucC, iutA, ompT, sitA |
| A41.2-1 | pZPK-A41.2-1 | IncFIB, p0111 | [F-:A-:B76] | 153291 | blaCTX–M–65 | floR, oqxA, oqxB, dfrA17, aac(3)-IV, aadA5, aph(4)-Ia, sul1, sul2 | papC |
| C51-1 | pZPK-C51-1 | IncI1-Iγ | 95 (CC-9) | 121837 | blaSHV–12 | aadA1, aadA2b, tet(A), sul3, cmlA1 | cib |
| C56.1-1 | pZPK-C56.1-1 | IncI1-Iγ | 36 (CC-3) | 89504 | blaTEM–52C | – | – |
| C61-1 | pZPK-C61-1 | IncI1-Iγ | 2 (CC-2) | 97275 | blaCMY–2 | – | cia |
| C81.1-1 | pZPK-C81.1-1 | IncI1-Iγ | 3 (CC-3) | 112374 | blaCTX–M–1 | – | cib |
| C86.1-1 | pZPK-C86.1-1_X1 | IncX1 | – | 47686 | blaTEM–1B | qnrS1 |
aAnalysis tools by Center for Genomic Epidemiology: ResFinder 3.2 for acquired resistance genes; PlasmidFinder 2.1 for determining beta-lactamase harboring plasmid replicons; pMLST 2.0 for plasmid sequence type (ST) (CC, clonal complex; pMLST, plasmid multilocus sequence typing; RST, replicon sequence typing).