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. 2021 Feb 3;11:592080. doi: 10.3389/fimmu.2020.592080

Figure 2.

Figure 2

The transfected efficient of Sox9-KD lentivirus in U251, U373, and U87 cells were detected by GFP stain. (A) U251, U373, and U87 Sox9-kd stable cell lines were detected by Western blot. (B) CCK8 assay of both U251 Sox9-kd cells and U251 Sox9-nc cells, as well as U373 Sox9-kd cells and U373 Sox9-nc cells in 24, 48, and 72 h (n = 3, p < 0.001). (C) Wound healing assay of both U251 Sox9-kd cells and U251 Sox9-nc cells at 0, 24 (p < 0.01), and 48 h (p < 0.005), as well as U373 Sox9-kd cells and U373 Sox9-nc cells (24 h: p < 0.05; 48 h: p < 0.005). (D) Transwell (migration) assay in U251 Sox9-kd cells and U251 Sox9-nc cells (p < 0.005), as well as in U373 Sox9-kd cells and U373 Sox9-nc cells (p < 0.001). (E) Transwell (invasion) assay in U251 Sox9-kd cells and U251 Sox9-nc cells (p < 0.001), as well as in U373 Sox9-kd cells and U373 Sox9-nc cells (p < 0.001). (F) Tumorigenesis xenografts with U87 Sox9-nc cells and U87 Sox9- kd cells after 40 days (n = 10). (G) Statistical analysis was performed using a two-tailed independent t-test. Values of p < 0.05 (*), p < 0.01 (**), p < 0.005 (***), and p < 0.001 (****) were considered statistically significant.