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. 2021 Feb 3;11:592080. doi: 10.3389/fimmu.2020.592080

Figure 4.

Figure 4

Expression of Sox9 was detected by western blot after that U251 cells and U373 cells were treated with LY2109761 (5 μM) at 12 h, and then while Sox9 was overexpressed (OE). (A) CCK8 assay among DMSO-treated U251 cells, LY2109761-treated U251 cells, and LY2109761+Sox9-OE-treated U251 cells, as well as in U373 cells (LY2109763 vs LY2109763+Sox9-OE: ns). (B) Wound-healing assay of DMSO-treated U251 cells, LY2109761-treated U251 cells, and LY2109761+Sox9-OE-treated U251 cells at 0, 24 (LY2109763 vs LY2109763+Sox9-OE: p < 0.005), and 48 h (LY2109763 vs LY2109763+Sox9-OE: p < 0.005), as well as U373 cells (LY2109763 vs LY2109763+Sox9-OE: 24 h: p < 0.0.01; 48 h: p < 0.05). (C) Transwell (migration) assay in among DMSO-treated U251 cells, LY2109761-treated U251 cells, and LY2109761+Sox9-OE-treated U251 cells (LY2109763 vs LY2109763+Sox9-OE: p < 0.005), as well as in U373 cells (LY2109763 vs LY2109763+Sox9-OE: p < 0.001). (D) Transwell (invasion) assay in among DMSO-treated U251 cells, LY2109761-treated U251 cells, and LY2109761+Sox9-OE-treated U251 cells (LY2109763 vs LY2109763+Sox9-OE: p < 0.005), as well as in U373 cells (LY2109763 vs LY2109763+Sox9-OE: p < 0.001). (E) Statistical analysis was performed using a two-tailed independent t-test. Values of p < 0.05 (*), p < 0.01 (**), p < 0.005 (***), and p < 0.001 (****) were considered statistically significant.