Fig. 6. Primitive subtype is more sensitive to kinase inhibitors.

Activity of three different kinases inhibitors Sorafenib, Sunitinib, and Ruxolitinib is shown in UHN and BeatAML datasets. Inhibitors were assessed against samples from primitive and committed subtype in patient derived cells (ex vivo drug screening). Top panel shows drug-dose response curves for individual patient samples (in dotted lines) and average across subtypes (in solid line) in the UHN cohort. Lines in red color indicate primitive subtype and blue color indicate committed subtype. The second and third panels show AUC values for ex vivo drug screening in the UHN and BeatAML cohort, respectively. Boxplots in red indicate primitive and in blue indicate committed subtype. Wilcoxon rank-sum test-based p-values are indicated on the top of box plots. Samples within the primitive subtype show higher sensitivity (two-sided Wilcoxon rank-sum test p < 0.05) against inhibitors Sorafenib, Sunitinib, and Ruxolitinib in the UHN dataset. In the validation cohort (BeatAML), the primitive subtype shows higher sensitivity against Sorafenib and Sunitinib (two-sided Wilcoxon rank-sum test p < 0.05).