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. 2021 Feb 16;4:218. doi: 10.1038/s42003-021-01711-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Distribution of carbon atoms present in FA chains of a all PL species, b PC species, and c GL species from Jurkat solubilized membranes and membrane pellet. The PC length profiles observed for the solubilized samples largely resemble to that of the membrane, with some differences observed for the detergent sample that was enriched in PC species with 4–6 and ≥8 double bonds and depleted of 1–2 and 7 double bonds. Contrarily, the unmodified SMA samples were enriched in 6 and more double bond PC species and SMA(2.3:1) depleted of saturated and monounsaturated PC lipids. In general, the differences seen in total PL are therefore a result of the distinct headgroup composition of the samples. Data points correspond to three technical replicates, except for technical duplicates for SMA-QA. Error bars represent ±S.D. Significant differences (upon one-way ANOVA) are denoted as *p < 0.05, **p < 0.01, ***p < 0.001.