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. 2021 Feb 16;11:3878. doi: 10.1038/s41598-020-80587-5

Figure 7.

Figure 7

Effects of PACs on insulin sensitivity in Caco-2/15 cell line. Proanthocyanidins (PACs, 250 µg/mL) were added to the apical cell compartment for 24 h prior to treatment with Fe/Asc (200 µM/2 mM) for 6 h at 37 °C. Two hours before the end of the 6 h-incubation with Fe/Asc mixture, insulin (100 nM) was added to evaluate insulin sensitivity. The protein expression of (A) p38-MAPK, (B) phospho p38-MAPK, (D) Akt, (E) phospho Akt, (G) PI3k and (H) phospho PI3k was evaluated by Western blot. The ratios (C) phospho p38-MAPK/p38-MAPK, (F) phospho Akt/Akt and (I) phospho PI3k/PI3k were then calculated. Results represent the means ± SEM of 3 independent experiments each in duplicate. A representative Western blot is shown, illustrating an experiment in triplicate on the same gel and with the same exposure. *P < 0.05 vs +/− insulin untreated cells (ctrl). #P < 0.05 vs Fe/Asc +/− insulin treated cells. p38-MAPK p38-mitogen activated protein kinase, PI3k phosphoinositide 3-kinase, Akt protein kinase B.