Figure 2.

Immunization of mCD40-LMP1 Tg mice with EBNA-1, Sm, or controls to assess antigen-specific immune response and antigen cross-reactivity. As described in Materials and Methods, mCD40-LMP1 Tg, mCD40 WT (C57BL/6 and mCD40 Tg), and mCD40^-/- mice remained naïve or were immunized with either saline (adjuvant only), EBNA-1, or Sm on Day 0 in CFA, then received boost injections (in IFA) on Days 10 and Day 28. Mice were euthanized on Day 56, spleen and lymph nodes removed, and cell cultures completed. Blood samples were procured for serum prior to initial immunization (Day -3), and on Days 10, 28, and 56 after initial immunization. Separate groups of EBNA-1 immunized mice completed Days 0–10 or Days 0–28 of the protocol. Each experimental group contained 6–8 mice across two separate experiments.