Figure 3.

Enhanced antigen recall response to EBNA-1 and homologous region PPPGRRP in mCD40-LMP1 Tg mice. Lymph node cells (4x 10⁶/ml) were cultured in the presence of 50 µg/ml EBNA-1 (mosaic) vs. 50 µg/ml PPPGRRP (GRR; EBNA-1 antigenic epitope). Cell cultures were assessed for proliferation (A) and cell culture supernatant IL-17A (B), IFN-γ (C), IL-10 (D), IL-6 (E), and TNF-α (F). Antigen recall response from mCD40-LMP1 Tg mice was compared to that of CD40 WT mice and CD40^-/- mice ( Figure 2 ). Data presented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p < 0.001, ****p < 0.0001 one way ANOVA with Dunnett’s multiple comparison test. Significance between experimental groups of mice designated above bar graphs (mCD40-LMP1 mice over time) and below bar graphs (mCD40-LMP1 mice vs. CD40 WT vs. CD40^-/- mice); purple = EBNA-1; orange = GRR. A minimal cellular response was exhibited by naïve/adjuvant control mice (dotted line near bottom of y-axis). A minimal cellular response was exhibited by d0-10 and d0-28 mCD40 WT and CD40-deficient mice; only d0-56 data are shown. Antigenic stimulation vs. medium only and PMA/ionomycin is presented in Supplementary Figure 1 .