Fig. 2.

Yeast cells can acquire multiple mutations simultaneously. A) a cartoon illustrating a burst of mitotic recombination leading to multiple new LOH events. Shown are two pairs of homologous chromosomes (upper cell) and a derivative clone harboring numerous tracts of LOH (lower cell, arrowheads). B) Representative schematics of the parental diploid yeast genome and four selected clones that acquired multiple unselected tracts of LOH (Sampaio, et al. 2020). Each square represents both homologs of the denoted chromosome. White squares represent chromosomes that maintained the parental configuration. Grey squares represent the chromosome harboring the selected mutation. Black squares represent chromosomes that had concomitantly acquired an unselected tract of LOH. C) A cartoon illustrating a how a burst of aneuploidization can produce a clone harboring multiple aneuploidies. D) Representative schematics of the parental yeast genome and four selected clones that had acquired multiple unselected aneuploidies(Heasley, et al. 2020). White squares represent chromosomes that maintained the parental configuration (i.e., one copy of each homolog). Grey squares represent the selected chromosome that was lost. Black squares represent chromosome pairs affected by unselected aneuploidy.