Table 2.

rhACE2 K_m, V_max, K_cat and K_cat/K_m values for the metabolism of Ang II vs. Ang III vs. Ang IV.

Substrate	Km (μM)	Vmax (nmol /30 min	Kcat (sec−1)	Kcat/Km (μm−1 sec−1)
Ang II	8.53 ±1.43	1.83 ± 0.23	7.82 ± 0.97	0.90 ± 0.08
Ang III	2.87 ± 0.45***	3.65 ± 0.56*	15.6 ± 2.4*	6.52 ± 1.05**
Ang IV	19.6 ± 0.97†	14.3 ± 0.44†	61.3 ± 1.3†	3.07 ± 0.10

^*p < 0.05 greater than Ang II;

^***p < 0.0001 less than Ang II;

^**p < 0.001 greater than Ang II and p < 0.01 greater than ang IV;

^†p < 0.0001 greater than Ang II and Ang III. Values are mean ± SEM, n=5.