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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Circ Heart Fail. 2021 Feb 1;14(2):e007279. doi: 10.1161/CIRCHEARTFAILURE.120.007279

Figure 6: Deletion of mineralocorticoid receptor in smooth muscle cell (SMC-MR-KO) improves capillary density and coronary flow reserve in pressure overload-induced heart failure.

Figure 6:

Results of quantitative RT-PCR for angiogenesis markers: (A) basic fibroblast growth factor (FGF2), (B) placental growth factor (PGF), and (C) vascular endothelial growth factor receptor 1 (FLT1). (D) Representative LV sections stained with CD31 to mark endothelial cells. Scale bar=50 μm. (E) Quantification of capillary density using CD31 stained area normalized to cardiomyocyte area. (F) Coronary flow reserve assessed by echocardiography before and after a hyperemic stimulus. A-C: MR-intact Sham n=3, MR-intact TAC n=6, SMC-MR-KO Sham n=3, SMC-MR-KO TAC n=6. E: MR-intact Sham n=3, MR-intact TAC n=11, SMC-MR-KO Sham n=3, SMC-MR-KO TAC n=5. F: MR-intact Sham n=6, MR-intact TAC n=16, SMC-MR-KO Sham n=7, SMC-MR-KO TAC n=13. Two-way ANOVA with Tukey post-hoc test.